STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)

ABSTRACT Pomegranate (Punica granatum L) is widely used for health. Pomegranate peel is part of the pomegranate fruit which is often discarded and has not been widely used. Pomegranate peel is thought to contain antioxidants. One of the examinations for antioxidants is the 1,1 dipheyl-2-picrylhydraz...

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Main Authors:	Wahyuni, Sri, Mahmuda, Iin Novita Nurhidayati, Maryati, Perdana, Aditya Putra, Azizah, Maharotullaili Nur
Format:	UMS Journal (OJS)
Language:	eng
Published:	Universitas Muhammadiyah Surakarta 2023
Subjects:	Pomegranate Peel Ethanol Extract Ethyl Acetate Fraction Isolate Antioxidant DPPH Kulit Delima Ekstrak Etanol Fraksi Etil Asetat Isolat Antioksidan
Online Access:	https://journals2.ums.ac.id/index.php/biomedika/article/view/1751
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author	Wahyuni, Sri Mahmuda, Iin Novita Nurhidayati Maryati Perdana, Aditya Putra Azizah, Maharotullaili Nur
author_facet	Wahyuni, Sri Mahmuda, Iin Novita Nurhidayati Maryati Perdana, Aditya Putra Azizah, Maharotullaili Nur
author_sort	Wahyuni, Sri
collection	OJS
description	ABSTRACT Pomegranate (Punica granatum L) is widely used for health. Pomegranate peel is part of the pomegranate fruit which is often discarded and has not been widely used. Pomegranate peel is thought to contain antioxidants. One of the examinations for antioxidants is the 1,1 dipheyl-2-picrylhydrazyl (DPPH) method, by calculating the IC50 value, where the smaller the IC50 value means that the stronger of antioxidant potential. The purpose of this study was to compare the antioxidant activity of the ethanol extract, ethyl acetate fraction, and the active isolate from pomegranate peel. The antioxidant potency was tested using the DPPH, using the UV-Vis spectrophotometer, at 515 nm wavelength. Isolation of isolates was carried out by column chromatography shepadex and TLC with toluene as mobile phase; ethyl acetate : formic acid : methanol, with a ratio of 3:3:0.8:0.2. The purity of the isolate was detected by HPLC, with water-methanol as the mobile phase. The research results showed that 96% ethanol extract, ethyl acetate fraction and active isolate from pomegranate peel have potential as antioxidants with IC50 4.52 ± 0.00, 2.71 ± 0.01, and 0.979 x 10-5 ± 0.00 µg/mL, respectively, the positive control was vitamin C, had an IC50 3.66 ± 0.02 µg/mL. The conclusion of this study is that pomegranate peel isolate has the strongest antioxidant potential, compared to 96% ethanol extract and the ethyl acetate fraction of pomegranate peel, and even vitamin C. ABSTRAK Delima (Punica granatum L) banyak dimanfaatkan untuk kesehatan. Kulit delima merupakan bagian dari delima yang sering kali dibuang dan belum banyak dimanfaatkan. Kulit delima diduga memiliki kandungan antioksidan. Salah satu pemeriksaan antioksidan adalah dengan metode 1,1 dipheyl-2-picrylhydrazyl (DPPH), dengan menghitung nilai IC50, dimana semakin kecil nilai IC50 dapat diartikan bahwa semakin kuat potensi antioksidannya. Tujuan dari penelitian ini adalah untuk membandingkan aktivitas antioksidan dari ektrak etanol, fraksi etil asetat, dan isolat aktif kulit delima. Potensi antioksidan diuji dengan metode DPPH, dengan menggunakan spektrofotometer UV-Vis, pada panjang gelombang 515 nm. Isolasi isolat dilakukan dengan kromatografi kolom shepadex dan KLT dengan fase gerak toluene: etil asetat: asam format: metanol, dengan perbandingan 3:3:0.8:0.2. Kemurnian isolat di deteksi dengan HPLC, dengan fase gerak air-metanol. Hasil peneiltian menunjukan bahwa ekstrak etanol 96%, fraksi etil asetat dan isolat aktif dari kulit delima memiliki potensi sebagai antioksidan, dengan nilai IC50 masing-masing sebesar 4,52 ± 0,00; 2,71 ± 0,01; dan 0,979 x 10-5 ± 0,00 µg/ mL, sebagai kontrol positif vitamin C memiliki nilai IC50 3,66 ± 0,02 µg/ mL. Kesimpulan penelitian ini adalah bahwa isolat kulit delima memiliki potensi antioksidan terkuat, dibandingkan ekstrak etanol 96% dan fraksi etil asetat kulit delima, bahkan juga vitamin C.
format	UMS Journal (OJS)
id	oai:ojs2.journals2.ums.ac.id:article-1751
institution	Universitas Muhammadiyah Surakarta
language	eng
publishDate	2023
publisher	Universitas Muhammadiyah Surakarta
record_format	ojs
spelling	oai:ojs2.journals2.ums.ac.id:article-1751 STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.) Wahyuni, Sri Mahmuda, Iin Novita Nurhidayati Maryati Perdana, Aditya Putra Azizah, Maharotullaili Nur Pomegranate Peel Ethanol Extract Ethyl Acetate Fraction Isolate Antioxidant DPPH Kulit Delima Ekstrak Etanol Fraksi Etil Asetat Isolat Antioksidan ABSTRACT Pomegranate (Punica granatum L) is widely used for health. Pomegranate peel is part of the pomegranate fruit which is often discarded and has not been widely used. Pomegranate peel is thought to contain antioxidants. One of the examinations for antioxidants is the 1,1 dipheyl-2-picrylhydrazyl (DPPH) method, by calculating the IC50 value, where the smaller the IC50 value means that the stronger of antioxidant potential. The purpose of this study was to compare the antioxidant activity of the ethanol extract, ethyl acetate fraction, and the active isolate from pomegranate peel. The antioxidant potency was tested using the DPPH, using the UV-Vis spectrophotometer, at 515 nm wavelength. Isolation of isolates was carried out by column chromatography shepadex and TLC with toluene as mobile phase; ethyl acetate : formic acid : methanol, with a ratio of 3:3:0.8:0.2. The purity of the isolate was detected by HPLC, with water-methanol as the mobile phase. The research results showed that 96% ethanol extract, ethyl acetate fraction and active isolate from pomegranate peel have potential as antioxidants with IC50 4.52 ± 0.00, 2.71 ± 0.01, and 0.979 x 10-5 ± 0.00 µg/mL, respectively, the positive control was vitamin C, had an IC50 3.66 ± 0.02 µg/mL. The conclusion of this study is that pomegranate peel isolate has the strongest antioxidant potential, compared to 96% ethanol extract and the ethyl acetate fraction of pomegranate peel, and even vitamin C. ABSTRAK Delima (Punica granatum L) banyak dimanfaatkan untuk kesehatan. Kulit delima merupakan bagian dari delima yang sering kali dibuang dan belum banyak dimanfaatkan. Kulit delima diduga memiliki kandungan antioksidan. Salah satu pemeriksaan antioksidan adalah dengan metode 1,1 dipheyl-2-picrylhydrazyl (DPPH), dengan menghitung nilai IC50, dimana semakin kecil nilai IC50 dapat diartikan bahwa semakin kuat potensi antioksidannya. Tujuan dari penelitian ini adalah untuk membandingkan aktivitas antioksidan dari ektrak etanol, fraksi etil asetat, dan isolat aktif kulit delima. Potensi antioksidan diuji dengan metode DPPH, dengan menggunakan spektrofotometer UV-Vis, pada panjang gelombang 515 nm. Isolasi isolat dilakukan dengan kromatografi kolom shepadex dan KLT dengan fase gerak toluene: etil asetat: asam format: metanol, dengan perbandingan 3:3:0.8:0.2. Kemurnian isolat di deteksi dengan HPLC, dengan fase gerak air-metanol. Hasil peneiltian menunjukan bahwa ekstrak etanol 96%, fraksi etil asetat dan isolat aktif dari kulit delima memiliki potensi sebagai antioksidan, dengan nilai IC50 masing-masing sebesar 4,52 ± 0,00; 2,71 ± 0,01; dan 0,979 x 10-5 ± 0,00 µg/ mL, sebagai kontrol positif vitamin C memiliki nilai IC50 3,66 ± 0,02 µg/ mL. Kesimpulan penelitian ini adalah bahwa isolat kulit delima memiliki potensi antioksidan terkuat, dibandingkan ekstrak etanol 96% dan fraksi etil asetat kulit delima, bahkan juga vitamin C. Universitas Muhammadiyah Surakarta 2023-02-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article application/pdf https://journals2.ums.ac.id/index.php/biomedika/article/view/1751 10.23917/biomedika.v15i1.1751 Biomedika; Vol 15, No 1 (2023): Biomedika Februari 2023; 67-75 2541-2582 2085-8345 eng https://journals2.ums.ac.id/index.php/biomedika/article/view/1751/531 https://creativecommons.org/licenses/by/4.0
spellingShingle	Pomegranate Peel Ethanol Extract Ethyl Acetate Fraction Isolate Antioxidant DPPH Kulit Delima Ekstrak Etanol Fraksi Etil Asetat Isolat Antioksidan Wahyuni, Sri Mahmuda, Iin Novita Nurhidayati Maryati Perdana, Aditya Putra Azizah, Maharotullaili Nur STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)
title	STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)
title_full	STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)
title_fullStr	STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)
title_full_unstemmed	STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)
title_short	STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)
title_sort	studi komparatif invitro aktivitas antioksidan ekstrak etanol fraksi etil asetat dan isolat aktif kulit delima punica granatum l in vitro comparative study antioxidant of ethanol extract ethyl acetate fraction and active isolate of pomegranate peel punica granatum l
topic	Pomegranate Peel Ethanol Extract Ethyl Acetate Fraction Isolate Antioxidant DPPH Kulit Delima Ekstrak Etanol Fraksi Etil Asetat Isolat Antioksidan
topic_facet	Pomegranate Peel Ethanol Extract Ethyl Acetate Fraction Isolate Antioxidant DPPH Kulit Delima Ekstrak Etanol Fraksi Etil Asetat Isolat Antioksidan
url	https://journals2.ums.ac.id/index.php/biomedika/article/view/1751
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STUDI KOMPARATIF INVITRO AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL, FRAKSI ETIL ASETAT DAN ISOLAT AKTIF KULIT DELIMA (PUNICA GRANATUM L.): In Vitro Comparative Study Antioxidant of Ethanol Extract, Ethyl Acetate Fraction And Active Isolate of Pomegranate Peel (Punica Granatum L.)

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