Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell
Avocado seeds, which often end up as food waste, have many health benefits, including anticancer activity. This study aimed to determine the potential cytotoxic activity of avocado seeds against breast cancer cells, MCF7. Avocado seeds were macerated with 96% ethanol and fractionated with n-hexane,...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | UMS Journal (OJS) |
| Language: | eng |
| Published: |
Universitas Muhammadiyah Surakarta
2022
|
| Subjects: | |
| Online Access: | https://journals.ums.ac.id/index.php/pharmacon/article/view/18274 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1805340888567644160 |
|---|---|
| author | Rahwawati, Juwita Maryati, Maryati Yuliani, Ratna |
| author_facet | Rahwawati, Juwita Maryati, Maryati Yuliani, Ratna |
| author_sort | Rahwawati, Juwita |
| collection | OJS |
| description | Avocado seeds, which often end up as food waste, have many health benefits, including anticancer activity. This study aimed to determine the potential cytotoxic activity of avocado seeds against breast cancer cells, MCF7. Avocado seeds were macerated with 96% ethanol and fractionated with n-hexane, chloroform, and ethyl acetate as solvents. Cytotoxic activity of the extract and fractions was tested using the MTT method, while antiproliferation activity and apoptosis of the n-hexane fraction were tested using the doubling time and double staining methods, respectively. The selectivity of the n-hexane fraction was also determined. The results showed that the extract and n-hexane fraction of avocado seeds had cytotoxic activity on MCF7 with IC50 values of 211.2±25 and 47.87±1.5 µg/mL, respectively. The cytotoxic activity of the n-hexane fraction was selective (Selectivity Index = 4.73). The fraction can extend the doubling time to 76.54-77.41 hours. The double staining test results showed red fluorescence in the cells, indicating the apoptosis of MCF7 cells. The n-hexane fraction of avocado seed extract has the potential to be developed as an anticancer therapeutic agent. |
| format | UMS Journal (OJS) |
| id | oai:ojs2.journals.ums.ac.id:article-18274 |
| institution | Universitas Muhammadiyah Surakarta |
| language | eng |
| publishDate | 2022 |
| publisher | Universitas Muhammadiyah Surakarta |
| record_format | ojs |
| spelling | oai:ojs2.journals.ums.ac.id:article-18274 Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell Rahwawati, Juwita Maryati, Maryati Yuliani, Ratna Persea americana Mill., MCF7, MTT, cytotoxic, apoptosis Avocado seeds, which often end up as food waste, have many health benefits, including anticancer activity. This study aimed to determine the potential cytotoxic activity of avocado seeds against breast cancer cells, MCF7. Avocado seeds were macerated with 96% ethanol and fractionated with n-hexane, chloroform, and ethyl acetate as solvents. Cytotoxic activity of the extract and fractions was tested using the MTT method, while antiproliferation activity and apoptosis of the n-hexane fraction were tested using the doubling time and double staining methods, respectively. The selectivity of the n-hexane fraction was also determined. The results showed that the extract and n-hexane fraction of avocado seeds had cytotoxic activity on MCF7 with IC50 values of 211.2±25 and 47.87±1.5 µg/mL, respectively. The cytotoxic activity of the n-hexane fraction was selective (Selectivity Index = 4.73). The fraction can extend the doubling time to 76.54-77.41 hours. The double staining test results showed red fluorescence in the cells, indicating the apoptosis of MCF7 cells. The n-hexane fraction of avocado seed extract has the potential to be developed as an anticancer therapeutic agent. Universitas Muhammadiyah Surakarta 2022-06-25 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://journals.ums.ac.id/index.php/pharmacon/article/view/18274 10.23917/pharmacon.v19i1.18274 Pharmacon: Jurnal Farmasi Indonesia; Vol 19, No 1 (2022); 35-44 2685-5062 1411-4283 eng https://journals.ums.ac.id/index.php/pharmacon/article/view/18274/7645 Copyright (c) 2022 Pharmacon: Jurnal Farmasi Indonesia https://creativecommons.org/licenses/by-nc-sa/4.0 |
| spellingShingle | Persea americana Mill., MCF7, MTT, cytotoxic, apoptosis Rahwawati, Juwita Maryati, Maryati Yuliani, Ratna Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell |
| title | Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell |
| title_full | Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell |
| title_fullStr | Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell |
| title_full_unstemmed | Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell |
| title_short | Cytotoxic and Antiproliferation Activity of n-Hexane Fraction of Avocado seed (Persea americana Mill.) on MCF7 cell |
| title_sort | cytotoxic and antiproliferation activity of n hexane fraction of avocado seed persea americana mill on mcf7 cell |
| topic | Persea americana Mill., MCF7, MTT, cytotoxic, apoptosis |
| topic_facet | Persea americana Mill., MCF7, MTT, cytotoxic, apoptosis |
| url | https://journals.ums.ac.id/index.php/pharmacon/article/view/18274 |
| work_keys_str_mv | AT rahwawatijuwita cytotoxicandantiproliferationactivityofnhexanefractionofavocadoseedperseaamericanamillonmcf7cell AT maryatimaryati cytotoxicandantiproliferationactivityofnhexanefractionofavocadoseedperseaamericanamillonmcf7cell AT yulianiratna cytotoxicandantiproliferationactivityofnhexanefractionofavocadoseedperseaamericanamillonmcf7cell |